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The Renin-Angiotensin-Aldosterone System

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Cover of 'The Renin-Angiotensin-Aldosterone System'

Table of Contents

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    Book Overview
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    Chapter 1 A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques
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    Chapter 2 A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining
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    Chapter 3 Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) Using Immunoblotting Approaches
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    Chapter 4 Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells
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    Chapter 5 Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta
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    Chapter 6 Measuring Blood Pressure Using a Noninvasive Tail Cuff Method in Mice
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    Chapter 7 Blood Pressure Monitoring Using Radio Telemetry Method in Mice
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    Chapter 8 Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry
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    Chapter 9 Assessment of the Renin–Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria
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    Chapter 10 Comprehensive Assessments of Energy Balance in Mice
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    Chapter 11 In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis
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    Chapter 12 A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt
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    Chapter 13 Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors
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    Chapter 14 A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection
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    Chapter 15 Analysis of Angiotensin Metabolism in the Kidney Using Mass Spectrometry
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    Chapter 16 Erratum to: Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry
Attention for Chapter 13: Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors
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Chapter title
Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors
Chapter number 13
Book title
The Renin-Angiotensin-Aldosterone System
Published in
Methods in molecular biology, May 2017
DOI 10.1007/978-1-4939-7030-8_13
Pubmed ID
Book ISBNs
978-1-4939-7028-5, 978-1-4939-7030-8
Authors

Maiia E. Bragina, Nikolaos Stergiopulos, Rodrigo A. Fraga-Silva

Editors

Sean E. Thatcher

Abstract

Binding assay is a common technique used to characterize ability of a ligand to interact with a specific biological target. A number of parameters, such as binding affinity, receptor density, and association/dissociation rate constants, can be measured by means of this technique. In most cases, implementation of the binding assay requires specific infrastructure for labeling and detecting the ligand, which impedes realization of this technique in a standard laboratory. Here we describe a simple fluorescence-based binding assay for angiotensin peptides and receptors, which does not require complex equipment and can be used for initial screening of the novel ligands or mutational studies.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Other 2 18%
Student > Doctoral Student 2 18%
Student > Master 2 18%
Student > Ph. D. Student 1 9%
Researcher 1 9%
Other 0 0%
Unknown 3 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 18%
Chemistry 2 18%
Nursing and Health Professions 2 18%
Environmental Science 1 9%
Medicine and Dentistry 1 9%
Other 1 9%
Unknown 2 18%