Chapter title |
Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography
|
---|---|
Chapter number | 2 |
Book title |
Zymography
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
Hidetaro Yasumitsu |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
To detect serine protease activity by zymography, casein and CBB stain have been used as a substrate and a detection procedure, respectively. Casein zymography has been using substrate concentration at 1 mg/mL and employing conventional CBB stain. Although ordinary casein zymography provides reproducible results, it has several disadvantages including time-consuming and relative low sensitivity. Improved casein zymography, RAMA casein zymography, is rapid and highly sensitive. RAMA casein zymography completes the detection process within 1 h after incubation and increases the sensitivity at least by tenfold. In addition to serine protease, the method also detects metalloprotease 7 (MMP7, Matrilysin) with high sensitivity. |
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Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 25% |
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Lecturer | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 3 | 38% |
Readers by discipline | Count | As % |
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Unknown | 3 | 38% |