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Mendeley readers
| Chapter title |
Two-Dimensional Zymography of Proteases from Steatotic Duck Liver
|
|---|---|
| Chapter number | 14 |
| Book title |
Zymography
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7111-4_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
| Authors |
Jeff Wilkesman, María Fernanda Padrón, Liliana Kurz, Hervé Rémignon |
| Editors |
Jeff Wilkesman, Liliana Kurz |
| Abstract |
Protease activity present in liver cells with steatosis can be electrophoretically characterized. Zymographic techniques allow semi-quantitative results, successfully detecting cathepsin and metalloprotease activity using polyacrylamide gels copolymerized with gelatin and quantified by densitometry. By using specific inhibitors, the identity of the proteases can be confirmed. 2D zymography allows the determination of both M r. and pI of the metalloprotease and cathepsin activity present in the homogenates. The analysis of liver proteases activities in force fed ducks may elucidate the mechanisms behind steatosis development. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 1 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 1 | 100% |