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Mendeley readers
Chapter title |
Detection of Guaiacol Peroxidase on Electrophoretic Gels
|
---|---|
Chapter number | 18 |
Book title |
Zymography
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Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
Diana Castro, Lellys M. Contreras, Liliana Kurz, Jeff Wilkesman |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
It is possible to analyze peroxidase (POD) from different vegetable sources by electrophoresis. Zymography, i.e., a SDS-PAGE method to detect enzyme activity, is used to specifically detect POD activity and to visualize the total protein profile. For this purpose, we describe how a radish homogenate is prepared and submitted first to electrophoresis, and then, the POD activity present in the gel is reactivated and selectively stained using guaiacol as substrate. After scanning the gel, the same gel is further stained with Coomassie blue to determine the whole protein profile of the sample. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 2 | 25% |
Student > Bachelor | 2 | 25% |
Professor | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 2 | 25% |
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Agricultural and Biological Sciences | 1 | 13% |
Engineering | 1 | 13% |
Unknown | 2 | 25% |