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| Chapter title |
Sequential Detection of Thermophilic Lipase and Protease by Zymography
|
|---|---|
| Chapter number | 25 |
| Book title |
Zymography
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7111-4_25 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
| Authors |
Liliana Kurz, Zully Hernández, Lellys M. Contreras, Jeff Wilkesman |
| Editors |
Jeff Wilkesman, Liliana Kurz |
| Abstract |
Lipase and protease present in cell-free fractions of thermophilic Bacillus sp. cultures were analyzed by polyacrylamide gel (PAG) electrophoresis. After run, the gel is electrotransferred to another PAG copolymerized with glycerol tributyrate, olive oil, and gelatin. This multi-substrate gel was incubated first for lipase detection, until bands appeared, and then stained with Coomassie for protease detection. Advantages of this sequential procedure are the detection of two different enzyme activities on a single PAG, beside time and resource saving. |