| Chapter title |
Preparation of a Three-Dimensional Full Thickness Skin Equivalent
|
|---|---|
| Chapter number | 14 |
| Book title |
3D Cell Culture
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-7021-6_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7019-3, 978-1-4939-7021-6
|
| Authors |
Christian Reuter, Heike Walles, Florian Groeber, Reuter, Christian, Walles, Heike, Groeber, Florian |
| Editors |
Zuzana Koledova |
| Abstract |
In vitro test systems are a promising alternative to animal models. Due to the use of human cells in a three-dimensional arrangement that allows cell-cell or cell-matrix interactions these models may be more predictive for the human situation compared to animal models or two-dimensional cell culture systems. Especially for dermatological research, skin models such as epidermal or full-thickness skin equivalents (FTSE) are used for different applications. Although epidermal models provide highly standardized conditions for risk assessment, FTSE facilitate a cellular crosstalk between the dermal and epidermal layer and thus can be used as more complex models for the investigation of processes such as wound healing, skin development, or infectious diseases. In this chapter, we describe the generation and culture of an FTSE, based on a collagen type I matrix and provide troubleshooting tips for commonly encountered technical problems. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 18% |
| Student > Master | 8 | 18% |
| Student > Bachelor | 8 | 18% |
| Student > Ph. D. Student | 4 | 9% |
| Unspecified | 3 | 7% |
| Other | 2 | 4% |
| Unknown | 12 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 24% |
| Agricultural and Biological Sciences | 5 | 11% |
| Unspecified | 4 | 9% |
| Immunology and Microbiology | 3 | 7% |
| Engineering | 2 | 4% |
| Other | 7 | 16% |
| Unknown | 13 | 29% |