| Chapter title |
Chromatin immunoprecipitation and chromatin immunoprecipitation with massively parallel sequencing on mouse embryonic tissue.
|
|---|---|
| Chapter number | 14 |
| Book title |
Hox Genes
|
| Published in |
Methods in molecular biology, July 2014
|
| DOI | 10.1007/978-1-4939-1242-1_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1241-4, 978-1-4939-1242-1
|
| Authors |
Shilu Amin, Nicoletta Bobola, Amin S, Bobola N, Amin, Shilu, Bobola, Nicoletta |
| Editors |
Yacine Graba, René Rezsohazy |
| Abstract |
Regulation of gene expression must be tightly controlled during embryonic development. A central mechanism to control gene expression is the binding of sequence-specific transcription factors to cis-regulatory elements in the genome. Chromatin immunoprecipitation (ChIP) is a widely used technique to analyze binding of transcription factors and histone modifications on chromatin; however, it is limited to looking at a small number of genes. ChIP with massively parallel sequencing (ChIP-seq) is a recently developed powerful tool to analyze genome-wide binding of transcription factors and histone modifications and provides a vast amount of information into the regulation of gene expression. This chapter describes how ChIP and ChIP-seq are performed on mouse embryonic tissue. |
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