| Chapter title |
Topological organization of Drosophila hox genes using DNA fluorescent in situ hybridization.
|
|---|---|
| Chapter number | 7 |
| Book title |
Hox Genes
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-1242-1_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1241-4, 978-1-4939-1242-1
|
| Authors |
Frédéric Bantignies, Giacomo Cavalli |
| Editors |
Yacine Graba, René Rezsohazy |
| Abstract |
DNA fluorescent in situ hybridization (FISH) is the method of choice to study genomic organization at the single-cell level. It has been recently used to study the topological organization of the homeotic bithorax complex (BX-C) in Drosophila as well as to describe long-range genomic interactions between the BX-C and the Antennapedia complex (ANT-C), in addition to other genomic loci. Coupled with immunofluorescence, FISH can be used to study the relative positioning of homeotic genes with nuclear subcompartments, such as Polycomb-group (PcG) bodies, transcription factories, or the nuclear lamina. Here, we describe two multicolor 3D-FISH protocols; one for whole mount Drosophila embryos or larval discs and one for Drosophila-cultured cells. Both methods can be applied to any single copy locus of interest and are compatible with immunostaining (FISH-I). |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 24% |
| Researcher | 4 | 24% |
| Student > Doctoral Student | 2 | 12% |
| Student > Master | 1 | 6% |
| Professor | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 5 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 35% |
| Biochemistry, Genetics and Molecular Biology | 5 | 29% |
| Materials Science | 1 | 6% |
| Unknown | 5 | 29% |