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Chapter title |
Bimolecular Fluorescence Complementation (BiFC) in Live Drosophila Embryos.
|
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Chapter number | 19 |
Book title |
Hox Genes
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Published in |
Methods in molecular biology, January 2014
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DOI | 10.1007/978-1-4939-1242-1_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1241-4, 978-1-4939-1242-1
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Authors |
Marilyne Duffraisse, Bruno Hudry, Samir Merabet |
Editors |
Yacine Graba, René Rezsohazy |
Abstract |
Bimolecular fluorescence complementation (BiFC) is a powerful method for studying protein-protein interactions in different cell types and organisms. This method was recently developed in the fruit fly Drosophila melanogaster, allowing analyzing protein interaction properties in a physiologically relevant developing context. Here we present a detailed protocol for performing BiFC with the Venus fluorescent protein in live Drosophila embryos, taking the Hox-PBC partnership as an illustrative test case. This protocol applies to any transcription factor and split fluorescent protein in general. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 33% |
Student > Doctoral Student | 3 | 25% |
Researcher | 2 | 17% |
Student > Bachelor | 1 | 8% |
Unknown | 2 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 50% |
Agricultural and Biological Sciences | 3 | 25% |
Chemistry | 1 | 8% |
Unknown | 2 | 17% |