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Chapter title |
Microinjection and Fluorescence In Situ Hybridization Assay for Studying mRNA Export in Mammalian Cells
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Chapter number | 8 |
Book title |
mRNA Processing
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7204-3_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7203-6, 978-1-4939-7204-3
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Authors |
Ke Wang, Min Shi, Hong Cheng |
Abstract |
Microinjection and Fluorescence in situ Hybridization (FISH) assay is a useful method for mRNA export studies, which can overcome the problems of traditional transfection in cells. Here, we describe the method of microinjection and FISH assay applied in investigation of mRNA export. By this method we can estimate the mRNA export kinetics, examining mRNA export in cells with low transfection efficiencies, and observing nuclear export of aberrant RNAs. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Unspecified | 1 | 50% |
Student > Ph. D. Student | 1 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 50% |