Chapter title |
Expansion of Human Mesenchymal Stem Cells in a Microcarrier Bioreactor
|
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Chapter number | 338 |
Book title |
Bioreactors in Stem Cell Biology
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_338 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
Authors |
Ang-Chen Tsai, Teng Ma |
Abstract |
Human mesenchymal stem cells (hMSCs) are considered as a primary candidate in cell therapy owing to their self-renewability, high differentiation capabilities, and secretions of trophic factors. In clinical application, a large quantity of therapeutically competent hMSCs is required that cannot be produced in conventional petri dish culture. Bioreactors are scalable and have the capacity to meet the production demand. Microcarrier suspension culture in stirred-tank bioreactors is the most widely used method to expand anchorage dependent cells in a large scale. Stirred-tank bioreactors have the potential to scale up and microcarriers provide the high surface-volume ratio. As a result, a spinner flask bioreactor with microcarriers has been commonly used in large scale expansion of adherent cells. This chapter describes a detailed culture protocol for hMSC expansion in a 125 mL spinner flask using microcarriers, Cytodex I, and a procedure for cell seeding, expansion, metabolic sampling, and quantification and visualization using microculture tetrazolium (MTT) reagent. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 54 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 9 | 17% |
Student > Master | 9 | 17% |
Student > Ph. D. Student | 7 | 13% |
Student > Bachelor | 5 | 9% |
Student > Doctoral Student | 2 | 4% |
Other | 4 | 7% |
Unknown | 18 | 33% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 6 | 11% |
Engineering | 5 | 9% |
Agricultural and Biological Sciences | 4 | 7% |
Chemical Engineering | 3 | 6% |
Other | 8 | 15% |
Unknown | 19 | 35% |