Chapter title |
Bioengineered Models of Solid Human Tumors for Cancer Research
|
---|---|
Chapter number | 353 |
Book title |
Bioreactors in Stem Cell Biology
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_353 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
Authors |
Alessandro Marturano-Kruik, Aranzazu Villasante, Gordana Vunjak-Novakovic |
Abstract |
The lack of controllable in vitro models that can recapitulate the features of solid tumors such as Ewing's sarcoma limits our understanding of the tumor initiation and progression and impedes the development of new therapies. Cancer research still relies of the use of simple cell culture, tumor spheroids, and small animals. Tissue-engineered tumor models are now being grown in vitro to mimic the actual tumors in patients. Recently, we have established a new protocol for bioengineering the Ewing's sarcoma, by infusing tumor cell aggregates into the human bone engineered from the patient's mesenchymal stem cells. The bone niche allows crosstalk between the tumor cells, osteoblasts and supporting cells of the bone, extracellular matrix, and the tissue microenvironment. The bioreactor platform used in these experiments also allows the implementation of physiologically relevant mechanical signals. Here, we describe a method to build an in vitro model of Ewing's sarcoma that mimics the key properties of the native tumor and provides the tissue context and physical regulatory signals. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 23% |
Student > Master | 4 | 15% |
Student > Bachelor | 3 | 12% |
Student > Postgraduate | 3 | 12% |
Student > Ph. D. Student | 3 | 12% |
Other | 5 | 19% |
Unknown | 2 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 27% |
Agricultural and Biological Sciences | 6 | 23% |
Chemical Engineering | 3 | 12% |
Engineering | 3 | 12% |
Medicine and Dentistry | 2 | 8% |
Other | 2 | 8% |
Unknown | 3 | 12% |