| Chapter title |
Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies
|
|---|---|
| Chapter number | 341 |
| Book title |
Bioreactors in Stem Cell Biology
|
| Published in |
Methods in molecular biology, April 2016
|
| DOI | 10.1007/7651_2016_341 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
| Authors |
Sasitorn Rungarunlert, Joao N. Ferreira, Andras Dinnyes |
| Abstract |
Generation of cardiomyocytes from pluripotent stem cells (PSCs) is a common and valuable approach to produce large amount of cells for various applications, including assays and models for drug development, cell-based therapies, and tissue engineering. All these applications would benefit from a reliable bioreactor-based methodology to consistently generate homogenous PSC-derived embryoid bodies (EBs) at a large scale, which can further undergo cardiomyogenic differentiation. The goal of this chapter is to describe a scalable method to consistently generate large amount of homogeneous and synchronized EBs from PSCs. This method utilizes a slow-turning lateral vessel bioreactor to direct the EB formation and their subsequent cardiomyogenic lineage differentiation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Lecturer | 2 | 18% |
| Other | 1 | 9% |
| Student > Master | 1 | 9% |
| Student > Doctoral Student | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 2 | 18% |
| Biochemistry, Genetics and Molecular Biology | 2 | 18% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Agricultural and Biological Sciences | 1 | 9% |
| Neuroscience | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 3 | 27% |