Chapter title |
Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading
|
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Chapter number | 332 |
Book title |
Bioreactors in Stem Cell Biology
|
Published in |
Methods in molecular biology, April 2016
|
DOI | 10.1007/7651_2016_332 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6476-5, 978-1-4939-6478-9
|
Authors |
Daniel W. Youngstrom, Jennifer G. Barrett |
Abstract |
Tendon bioreactors combine cells, scaffold, and mechanical stimulation to drive tissue neogenesis ex vivo. Faithful recapitulation of the native tendon microenvironment is essential for stimulating graft maturation or modeling tendon biology. As the mediator between cells and mechanical stimulation, the properties of a scaffold constitute perhaps the most essential elements in a bioreactor system. One method of achieving native scaffold properties is to process tendon allograft in a manner that removes cells without modifying structure and function: "decellularization." This chapter describes (1) production of tendon scaffolds derived from native extracellular matrix, (2) preparation of cell-laden scaffolds prior to bioreactor culture, and (3) tissue processing post-harvest for gene expression analysis. These methods may be applied for a variety of applications including graft production, cell priming prior to transplantation and basic investigations of tendon cell biology. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 33% |
Student > Master | 2 | 11% |
Professor | 2 | 11% |
Other | 1 | 6% |
Student > Bachelor | 1 | 6% |
Other | 2 | 11% |
Unknown | 4 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 22% |
Medicine and Dentistry | 3 | 17% |
Chemical Engineering | 2 | 11% |
Engineering | 2 | 11% |
Social Sciences | 1 | 6% |
Other | 1 | 6% |
Unknown | 5 | 28% |