Chapter title |
Hox transcriptomics in Drosophila embryos.
|
---|---|
Chapter number | 12 |
Book title |
Hox Genes
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1242-1_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1241-4, 978-1-4939-1242-1
|
Authors |
Maria Polychronidou, Ingrid Lohmann |
Editors |
Yacine Graba, René Rezsohazy |
Abstract |
Hox proteins are evolutionarily conserved homeodomain containing transcription factors that specify segment identities along the anteroposterior axis of almost all bilaterian animals. They exert their morphogenetic role by transcriptionally regulating a large battery of downstream target genes. Therefore the dissection of transcriptional networks regulated by Hox proteins is an essential step towards a mechanistic understanding of how these transcription factors coordinate multiple developmental and morphogenetic processes. High-throughput techniques allowing whole-transcriptome mRNA expression profiling are powerful tools for the genome-wide identification of Hox downstream target genes in a variety of experimental settings. Here, we describe how to quantitatively identify Hox downstream genes in Drosophila embryos by performing a Hox transcriptome analysis using microarrays. |
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