| Chapter title |
Knockdown of Rice microRNA166 by Short Tandem Target Mimic (STTM)
|
|---|---|
| Chapter number | 25 |
| Book title |
Functional Genomics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7231-9_25 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7230-2, 978-1-4939-7231-9
|
| Authors |
Sachin Teotia, Dabing Zhang, Guiliang Tang |
| Abstract |
Small RNAs, including microRNAs (miRNAs), are abundant in plants and play key roles in controlling plant development and physiology. miRNAs regulate the expression of the target genes involved in key plant processes. Due to functional redundancy among miRNA family members in plants, an ideal approach to silence the expression of all members simultaneously, for their functional characterization, is desirable. Target mimic (TM) was the first approach to achieve this goal. Short tandem target mimic (STTM) is a potent approach complementing TM for silencing miRNAs in plants. STTMs have been successfully used in dicots to block miRNA functions. Here, we describe in detail the protocol for designing STTM construct to block miRNA functions in rice. Such approach can be applied to silence miRNAs in other monocots as well. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 22% |
| Student > Doctoral Student | 1 | 11% |
| Other | 1 | 11% |
| Student > Master | 1 | 11% |
| Researcher | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Unknown | 4 | 44% |