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Mendeley readers
Chapter title |
Protein Arrays III: Reverse-Phase Protein Arrays
|
---|---|
Chapter number | 21 |
Book title |
Functional Genomics
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7231-9_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7230-2, 978-1-4939-7231-9
|
Authors |
Yulin Yuan, Xia Hong, Zuan-Tao Lin, Hongting Wang, Mikala Heon, Tianfu Wu |
Abstract |
The reverse-phase protein array (RPPA) is to use highly specific antibodies to interrogate pan or posttranslationally modified protein targets, such as phosphorylated proteins, particularly the proteins involved in cell signaling pathways. In this protocol we will cover the preparation of cell (or tissue) lysates, sample printing, antibody validation, antibody interrogation, signal amplification steps, imaging and data analysis. In this protocol, colorimetric catalyzed signal amplification (CSA) chemistry, fluorescence and near-infrared (NIR) based detection methods will be described. |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 41 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 7% |
Unknown | 38 | 93% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 5% |
Sports and Recreations | 1 | 2% |
Unknown | 38 | 93% |