Functional Genomics

Chapter 1 Predicting RNA Structure with Vfold

Chapter 2 RNA Function Prediction

Chapter 3 Computational Prediction of Novel miRNAs from Genome-Wide Data

Chapter 4 Protein Structure Modeling with MODELLER

Chapter 5 Protein Function Prediction

Chapter 6 Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C

Chapter 7 Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila

Chapter 8 DNA Methylation Profiling Using Long-Read Single Molecule Real-Time Bisulfite Sequencing (SMRT-BS)

Chapter 9 Copy Number Variation Analysis by Droplet Digital PCR

Chapter 10 MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein–Nucleic Acid Interactions in Solution

Chapter 11 Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging

Chapter 12 Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy

Chapter 13 MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation

Chapter 14 Using FirePlex™ Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification

Chapter 15 Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling

Chapter 16 Optimized Whole Transcriptome Profiling of Motor Axons

Chapter 17 2D-DIGE in Proteomics

Chapter 18 STAGE-Diging in Proteomics

Chapter 19 Protein Arrays I: Antibody Arrays

Chapter 20 Protein Arrays II: Antigen Arrays

Chapter 21 Protein Arrays III: Reverse-Phase Protein Arrays

Chapter 22 Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry

Chapter 23 Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the “One-Step” Bean pod mottle virus (BPMV) Viral Vector

Chapter 24 Re-expressing Epigenetically Silenced Genes by Inducing DNA Demethylation Through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus

Chapter 25 Knockdown of Rice microRNA166 by Short Tandem Target Mimic (STTM)

Chapter 26 RNAi-Mediated Knockdown of Protein Expression

Chapter 27 Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells

Chapter 28 Production, Purification, and Titration of First-Generation Adenovirus Vectors