Chapter title |
Analysis of Borrelia burgdorferi Proteome and Protein–Protein Interactions
|
---|---|
Chapter number | 19 |
Book title |
Borrelia burgdorferi
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7383-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7382-8, 978-1-4939-7383-5
|
Authors |
Xiuli Yang, Meghna Thakur, Juraj Koci, Alexis A. Smith, Preeti Singh, Xuran Zhuang, Kamoltip Promnares, Yan Wang, Ozlem Buyuktanir, Utpal Pal |
Abstract |
The proteome of Borrelia burgdorferi undergoes dynamic alterations as the microbe cycles through and persists in diverse host or vector environments. Therefore, studies of B. burgdorferi proteome and protein-protein interactions, which play central roles in biological processes in diverse organisms, are critical in understanding biology and infectivity of spirochetes. Here, we describe the proteomic analysis of B. burgdorferi by two-dimensional (2-D) gel electrophoresis followed by protein identification via liquid chromatography-mass spectrometry and database searching. We also describe assays for studying the interaction between borrelial proteins: a novel high-throughput luciferase assay, yeast two-hybrid assay, and a far-Western assay that are routinely used in our laboratories. |
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United States | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 12 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 4 | 33% |
Researcher | 3 | 25% |
Professor | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Unknown | 3 | 25% |
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Immunology and Microbiology | 4 | 33% |
Agricultural and Biological Sciences | 3 | 25% |
Mathematics | 1 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Unknown | 3 | 25% |