| Chapter title |
Phagocytosis Assays for Borrelia burgdorferi
|
|---|---|
| Chapter number | 22 |
| Book title |
Borrelia burgdorferi
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7383-5_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7382-8, 978-1-4939-7383-5
|
| Authors |
Juan Anguita, Ana Carreras-González, Nicolás Navasa |
| Abstract |
Phagocytosis of Borrelia burgdorferi, the causative agent of Lyme disease, is a poorly understood process, despite its importance during the host immune response to infection. Thus, macrophages infiltrate the infected tissues, including the base of the heart and phagocytose the spirochete, therefore contributing to their elimination from infected tissues and leading to inflammation. An impaired bacterial clearance will result in bacterial persistence that may interfere with normal physiology of the heart, such as electrical signals from the heart, resulting in an impaired coordination of the beating of the heart or "heart block." This chapter presents a protocol for establishing primary mouse macrophage cultures, a method for lentivirus silencing of primary cells, and a method for the in vitro study of macrophage phagocytosis of fluorescently labeled Borrelia burgdorferi. |
X Demographics
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|---|---|---|
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| United States | 1 | 50% |
Demographic breakdown
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|---|---|---|
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Mendeley readers
Geographical breakdown
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|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Master | 2 | 15% |
| Student > Ph. D. Student | 2 | 15% |
| Professor | 1 | 8% |
| Unknown | 5 | 38% |
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| Agricultural and Biological Sciences | 1 | 8% |
| Social Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 6 | 46% |