Chapter title |
Detection and Analysis of Non-vesicular Extracellular RNA
|
---|---|
Chapter number | 10 |
Book title |
Extracellular RNA
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7652-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7651-5, 978-1-4939-7652-2
|
Authors |
Juan Pablo Tosar, Alfonso Cayota, Tosar, Juan Pablo, Cayota, Alfonso |
Abstract |
Although extracellular vesicles (EVs) are by far the most studied carriers of extracellular small RNAs involved in cell-to-cell communication, most extracellular small RNAs are actually present as soluble vesicle-free supramolecular complexes. Several proteins have been described as the counterparts of these RNase-protected complexes. Here we describe a method for the purification and analysis of non-vesicular extracellular RNA derived from the conditioned media of mammalian cell culture. Focus on this fraction will increase our understanding on extracellular RNA biology, while serving as a source for biomarker discovery complementary to EVs. |
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