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Extracellular RNA

Overview of attention for book
Cover of 'Extracellular RNA'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Extracellular RNAs: A New Awareness of Old Perspectives
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    Chapter 2 Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
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    Chapter 3 Extracellular RNA Isolation from Cell Culture Supernatant
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    Chapter 4 Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
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    Chapter 5 Isolation of Extracellular RNA from Serum/Plasma
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    Chapter 6 Isolation of Extracellular RNA from Bile
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    Chapter 7 Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes
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    Chapter 8 Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
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    Chapter 9 Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography
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    Chapter 10 Detection and Analysis of Non-vesicular Extracellular RNA
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    Chapter 11 Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
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    Chapter 12 Droplet Digital PCR for Quantitation of Extracellular RNA
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    Chapter 13 Preparation of Small RNA NGS Libraries from Biofluids
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    Chapter 14 Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString
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    Chapter 15 Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
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    Chapter 16 Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Attention for Chapter 2: Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
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  • Good Attention Score compared to outputs of the same age and source (66th percentile)

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Chapter title
Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
Chapter number 2
Book title
Extracellular RNA
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7652-2_2
Pubmed ID
Book ISBNs
978-1-4939-7651-5, 978-1-4939-7652-2
Authors

Julia Small, Roger Alexander, Leonora Balaj, Sabrina Roy

Abstract

Understanding the role of extracellular RNA (exRNA) has emerged as an exciting avenue for biomarker, therapeutic, as well as basic cell-cell communication applications and discoveries. Multiple protocols, kits, and procedures have been developed in the last decade to allow fractionation as well as isolation of subpopulations of macromolecules of interest found in biofluids. Here, we introduce the protocols decision tree developed by the Extracellular RNA Communication Consortium and available on their website (exRNA portal), and compare all methods currently available to the exRNA field and report pros and cons for each platform.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 27%
Student > Doctoral Student 3 14%
Professor > Associate Professor 2 9%
Student > Master 1 5%
Student > Bachelor 1 5%
Other 2 9%
Unknown 7 32%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 32%
Medicine and Dentistry 3 14%
Agricultural and Biological Sciences 2 9%
Immunology and Microbiology 2 9%
Chemical Engineering 1 5%
Other 1 5%
Unknown 6 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2020.
All research outputs
#15,962,021
of 25,257,066 outputs
Outputs from Methods in molecular biology
#4,721
of 14,164 outputs
Outputs of similar age
#255,159
of 455,677 outputs
Outputs of similar age from Methods in molecular biology
#445
of 1,486 outputs
Altmetric has tracked 25,257,066 research outputs across all sources so far. This one is in the 34th percentile – i.e., 34% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,164 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 455,677 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,486 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.