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Extracellular RNA

Overview of attention for book
Cover of 'Extracellular RNA'

Table of Contents

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    Book Overview
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    Chapter 1 Extracellular RNAs: A New Awareness of Old Perspectives
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    Chapter 2 Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles
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    Chapter 3 Extracellular RNA Isolation from Cell Culture Supernatant
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    Chapter 4 Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture
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    Chapter 5 Isolation of Extracellular RNA from Serum/Plasma
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    Chapter 6 Isolation of Extracellular RNA from Bile
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    Chapter 7 Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes
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    Chapter 8 Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids
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    Chapter 9 Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography
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    Chapter 10 Detection and Analysis of Non-vesicular Extracellular RNA
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    Chapter 11 Isolation of Plasma Lipoproteins as a Source of Extracellular RNA
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    Chapter 12 Droplet Digital PCR for Quantitation of Extracellular RNA
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    Chapter 13 Preparation of Small RNA NGS Libraries from Biofluids
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    Chapter 14 Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString
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    Chapter 15 Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs
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    Chapter 16 Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Attention for Chapter 16: Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
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Chapter title
Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs
Chapter number 16
Book title
Extracellular RNA
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7652-2_16
Pubmed ID
29388146
Book ISBNs
978-1-4939-7651-5, 978-1-4939-7652-2
Authors

Marie-Cecile Didiot, Reka A. Haraszti, Neil Aronin, Anastasia Khvorova

Abstract

Delivery represents a significant barrier to the clinical advancement of oligonucleotide therapeutics. Small, endogenous extracellular vesicles (EVs) have the potential to act as oligonucleotide delivery vehicles, but robust and scalable methods for loading RNA therapeutic cargo into vesicles are lacking. Here we describe the efficient loading of hydrophobically modified siRNAs (hsiRNAs) into EVs upon co-incubation, without altering vesicle size distribution or integrity. This method is expected to advance the development of EV-based therapies for the treatment of a broad range of disorders.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 27 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 30%
Researcher 3 11%
Student > Doctoral Student 2 7%
Student > Bachelor 2 7%
Professor 2 7%
Other 3 11%
Unknown 7 26%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 30%
Medicine and Dentistry 4 15%
Agricultural and Biological Sciences 3 11%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Chemistry 1 4%
Other 1 4%
Unknown 8 30%

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