Microfluidic devices for imaging trafficking events in vivo using genetic model organisms.
Exocytosis and Endocytosis
Methods in molecular biology, January 2014
Sudip Mondal, Sandhya P Koushika, Sandhya P. Koushika
Miniature devices are powerful new tools that can be used to address multiple questions in biology especially in investigating an individual cell or organism. The primary step forward has been the ease of soft lithography fabrication which has allowed researchers from different disciplines, with incomplete technical knowledge, to develop and use new devices for their own research problems. In this chapter, we describe a simple fabrication process that will allow investigators to make microfluidic devices for in vivo imaging studies using genetic model organisms such as C. elegans, Drosophila larvae, and zebrafish larvae. This microfluidic technology enables detailed studies on multiple cellular and subcellular phenomena including intracellular vesicle trafficking in living organisms over different developmental stages in an anesthetic free environment.
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