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Exocytosis and Endocytosis

Overview of attention for book
Cover of 'Exocytosis and Endocytosis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Pharmacological Inhibitors of Exocytosis and Endocytosis: Novel Bullets for Old Targets
  3. Altmetric Badge
    Chapter 2 Systematic Analysis of Endocytosis by Cellular Perturbations
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    Chapter 3 Real-Time Detection of SNARE Complex Assembly with FRET Using the Tetracysteine System
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    Chapter 4 Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides
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    Chapter 5 Secretion of circular proteins using sortase.
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    Chapter 6 Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-epithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation
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    Chapter 7 Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.
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    Chapter 8 Probabilistic Density Maps to Study the Spatial Organization of Endocytosis
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    Chapter 9 Use of Kaede and Kikume Green–Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors
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    Chapter 10 HaloTag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells
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    Chapter 11 SNAP-Tag to Monitor Trafficking of Membrane Proteins in Polarized Epithelial Cells
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    Chapter 12 FlAsH-PALM: Super-resolution Pointillist Imaging with FlAsH-Tetracysteine Labeling
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    Chapter 13 Analysis of protein dynamics with tandem fluorescent protein timers.
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    Chapter 14 Synchronization of Secretory Cargos Trafficking in Populations of Cells
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    Chapter 15 Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins
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    Chapter 16 A Method to Rapidly Induce Organelle-Specific Molecular Activities and Membrane Tethering
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    Chapter 17 A Novel Pair of Split Venus Fragments to Detect Protein–Protein Interactions by In Vitro and In Vivo Bimolecular Fluorescence Complementation Assays
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    Chapter 18 Real-Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy
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    Chapter 19 Nanocones to Study Initial Steps of Endocytosis
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    Chapter 20 A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography
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    Chapter 21 VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy
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    Chapter 22 Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using the Endomembrane Trafficking Network
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    Chapter 23 Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans
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    Chapter 24 Visualization of clathrin-mediated endocytosis in live Drosophila egg chambers.
  26. Altmetric Badge
    Chapter 25 A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling.
  27. Altmetric Badge
    Chapter 26 Microfluidic devices for imaging trafficking events in vivo using genetic model organisms.
  28. Altmetric Badge
    Chapter 27 The “In Situ” Proximity Ligation Assay to Probe Protein–Protein Interactions in Intact Tissues
  29. Altmetric Badge
    Chapter 28 Exocytosis and Endocytosis
  30. Altmetric Badge
    Chapter 29 Measurement of Dynamic F-Actin Changes During Exocytosis
Attention for Chapter 13: Analysis of protein dynamics with tandem fluorescent protein timers.
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Chapter title
Analysis of protein dynamics with tandem fluorescent protein timers.
Chapter number 13
Book title
Exocytosis and Endocytosis
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-4939-0944-5_13
Pubmed ID
Book ISBNs
978-1-4939-0943-8, 978-1-4939-0944-5
Authors

Anton Khmelinskii, Michael Knop

Abstract

Fluorescent timers (FTs) are fluorescent proteins that change color with time. FTs can be used as tags to follow protein dynamics in living cells. Recently we described a novel class of FTs called tandem fluorescent protein timers (tFTs). Each tFT is a tandem fusion of two different conventional fluorescent proteins having distinct kinetics of fluorophore maturation. tFTs suitable for studying protein dynamics on different scales can be generated from a broad range of commonly used fluorescent proteins. Here we describe how to establish new tFTs and consider potential pitfalls. We detail a protocol for quantitative fluorescence microscopy imaging and analysis of intracellular protein dynamics with tFTs in the budding yeast Saccharomyces cerevisiae.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Israel 1 3%
Germany 1 3%
Unknown 32 94%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 21%
Researcher 7 21%
Student > Ph. D. Student 6 18%
Student > Master 4 12%
Student > Doctoral Student 2 6%
Other 5 15%
Unknown 3 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 47%
Biochemistry, Genetics and Molecular Biology 12 35%
Chemical Engineering 1 3%
Immunology and Microbiology 1 3%
Unknown 4 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2014.
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#3,133,306
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Outputs from Methods in molecular biology
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#77,703
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Outputs of similar age from Methods in molecular biology
#73
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