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Mammary Stem Cells

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Cover of 'Mammary Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Breast Cancer Stem Cells: Current Advances and Clinical Implications
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    Chapter 2 A Protocol for Studying Embryonic Mammary Progenitor Cells During Mouse Mammary Primordial Development in Explant Culture
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    Chapter 3 FACS Sorting Mammary Stem Cells
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    Chapter 4 Side Population
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    Chapter 5 Single-cell genome and transcriptome processing prior to high-throughput sequencing.
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    Chapter 6 Shotgun proteomics on tissue specimens extracted with Acid guanidinium-thiocyanate-phenol-chloroform.
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    Chapter 7 Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments
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    Chapter 8 Lentiviral Transduction of Mammary Epithelial Cells
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    Chapter 9 The Transplantation of Mouse Mammary Epithelial Cells into Cleared Mammary Fat Pads
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    Chapter 10 Humanization of the Mouse Mammary Gland
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    Chapter 11 Lineage Tracing in the Mammary Gland Using Cre/lox Technology and Fluorescent Reporter Alleles
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    Chapter 12 Modeling the Breast Cancer Bone Metastatic Niche in Complex Three-Dimensional Cocultures
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    Chapter 13 Mammary Cancer Stem Cells Reinitiation Assessment at the Metastatic Niche: The Lung and Bone
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    Chapter 14 Nanomechanical Characterization of Living Mammary Tissues by Atomic Force Microscopy
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    Chapter 15 Mathematical Modelling as a Tool to Understand Cell Self-renewal and Differentiation
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    Chapter 16 Mammary Stem Cells: A Clinician’s View
Attention for Chapter 7: Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments
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Chapter title
Antibody-Based Capture of Target Peptides in Multiple Reaction Monitoring Experiments
Chapter number 7
Book title
Mammary Stem Cells
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2519-3_7
Pubmed ID
Book ISBNs
978-1-4939-2518-6, 978-1-4939-2519-3
Authors

Tommaso De Marchi, Eric Kuhn, Steven A. Carr, Arzu Umar

Abstract

Targeted quantitative mass spectrometry of immunoaffinity-enriched peptides, termed immuno-multiple reaction monitoring (iMRM), is a powerful method for determining the relative abundance of proteins in complex mixtures, like plasma or whole tissue. This technique combines 1,000-fold enrichment potential of antibodies for target peptides with the selectivity of multiple reaction monitoring mass spectrometry (MRM-MS). Using heavy isotope-labeled peptide counterparts as internal standards ensures high levels of precision. Further, LC-MRM-MS selectivity allows for multiplexing; antibodies recognizing different peptides can be added directly to a single mixture without subjecting to interferences common to other multiple antibody protein assays. Integrated extracted ion chromatograms (XIC) of product ions from endogenous unlabeled "light" peptide and stable isotope-labeled internal standard "heavy" peptides are used to generate a light/heavy peak area ratio. This ratio is proportional to the amount of peptide in the digestion mixture and can be used to estimate the concentration of protein in the sample.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 10%
Unknown 9 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 40%
Student > Bachelor 2 20%
Student > Postgraduate 2 20%
Researcher 1 10%
Student > Master 1 10%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 30%
Agricultural and Biological Sciences 3 30%
Medicine and Dentistry 2 20%
Engineering 1 10%
Unknown 1 10%