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Mendeley readers
Chapter title |
Lentiviral Transduction of Mammary Epithelial Cells
|
---|---|
Chapter number | 8 |
Book title |
Mammary Stem Cells
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2519-3_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2518-6, 978-1-4939-2519-3
|
Authors |
Richard Iggo, Elodie Richard |
Abstract |
Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including nondividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors, greatly facilitating studies on malignant transformation. We present simple protocols to produce and titrate lentiviral vectors, infect mammary epithelial cells, and check for contamination with replication-competent viruses. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 29% |
Student > Ph. D. Student | 2 | 14% |
Researcher | 1 | 7% |
Professor > Associate Professor | 1 | 7% |
Unknown | 6 | 43% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 21% |
Biochemistry, Genetics and Molecular Biology | 2 | 14% |
Arts and Humanities | 1 | 7% |
Psychology | 1 | 7% |
Unknown | 7 | 50% |