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Organoids

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Cover of 'Organoids'

Table of Contents

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    Book Overview
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    Chapter 1 New Trends and Perspectives in the Function of Non-neuronal Acetylcholine in Crypt–Villus Organoids in Mice
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    Chapter 1 Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections
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    Chapter 2 Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension
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    Chapter 2 A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment. - PubMed - NCBI
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    Chapter 3 Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix
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    Chapter 4 Oncogenic Transformation of Human-Derived Gastric Organoids
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    Chapter 5 Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres
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    Chapter 6 Study Bacteria–Host Interactions Using Intestinal Organoids
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    Chapter 7 Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System.
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    Chapter 8 Murine Colonic Organoid Culture System and Downstream Assay Applications.
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    Chapter 9 Construction of Thymus Organoids from Decellularized Thymus Scaffolds
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    Chapter 10 Drug Sensitivity Assays of Human Cancer Organoid Cultures
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    Chapter 11 Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells.
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    Chapter 12 Intestinal Organoids as a Novel Tool to Study Microbes–Epithelium Interactions
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    Chapter 13 Organoid Culture of Human Cancer Stem Cells
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    Chapter 14 Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-recombinase-Mediated Gene Deletion.
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    Chapter 15 The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
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    Chapter 21 Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation
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    Chapter 22 Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo
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    Chapter 46 Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering
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    Chapter 47 Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids
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    Chapter 48 Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells
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    Chapter 57 Gastrointestinal Epithelial Organoid Cultures from Postsurgical Tissues
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    Chapter 58 Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying
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    Chapter 68 Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly
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    Chapter 69 The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism
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    Chapter 70 Efficient Culture of Intestinal Organoids with Blebbistatin
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    Chapter 95 Clinically Amendable, Defined, and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells
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    Chapter 138 Erratum to: Drug Sensitivity Assays of Human Cancer Organoid Cultures
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    Chapter 169 Correction to: The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
Attention for Chapter 2: Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension
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Chapter title
Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension
Chapter number 2
Book title
Organoids
Published in
Methods in molecular biology, August 2016
DOI 10.1007/7651_2016_2
Pubmed ID
Book ISBNs
978-1-4939-7616-4, 978-1-4939-7617-1
Authors

Benedetti, Valentina, Brizi, Valerio, Xinaris, Christodoulos

Abstract

Novel methods in developmental biology and stem cell research have made it possible to generate complex kidney tissues in vitro that resemble whole organs and are termed organoids. In this chapter we describe a technique using suspensions of fully dissociated mouse kidney cells to yield organoids that can become vascularized in vivo and mature and display physiological functions. This system can be used to produce fine-grained human-mouse chimeric organoids in which the renal differentiation potential of human cells can be assessed. It can also be an excellent method for growing chimeric organoids in vivo using human stem cells, which can differentiate into specialized kidney cells and exert nephron-specific functions. We provide detailed methods, a brief discussion of critical points, and describe some successfully implemented examples of the system.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 31%
Student > Ph. D. Student 3 23%
Student > Master 2 15%
Student > Bachelor 1 8%
Student > Postgraduate 1 8%
Other 0 0%
Unknown 2 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 54%
Agricultural and Biological Sciences 2 15%
Medicine and Dentistry 2 15%
Unknown 2 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2016.
All research outputs
#20,337,788
of 22,883,326 outputs
Outputs from Methods in molecular biology
#9,920
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Outputs of similar age
#299,792
of 343,547 outputs
Outputs of similar age from Methods in molecular biology
#12
of 12 outputs
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So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.