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Organoids

Overview of attention for book
Cover of 'Organoids'

Table of Contents

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    Book Overview
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    Chapter 1 New Trends and Perspectives in the Function of Non-neuronal Acetylcholine in Crypt–Villus Organoids in Mice
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    Chapter 1 Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections
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    Chapter 2 Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension
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    Chapter 2 A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment. - PubMed - NCBI
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    Chapter 3 Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix
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    Chapter 4 Oncogenic Transformation of Human-Derived Gastric Organoids
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    Chapter 5 Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres
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    Chapter 6 Study Bacteria–Host Interactions Using Intestinal Organoids
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    Chapter 7 Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System.
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    Chapter 8 Murine Colonic Organoid Culture System and Downstream Assay Applications.
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    Chapter 9 Construction of Thymus Organoids from Decellularized Thymus Scaffolds
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    Chapter 10 Drug Sensitivity Assays of Human Cancer Organoid Cultures
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    Chapter 11 Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells.
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    Chapter 12 Intestinal Organoids as a Novel Tool to Study Microbes–Epithelium Interactions
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    Chapter 13 Organoid Culture of Human Cancer Stem Cells
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    Chapter 14 Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-recombinase-Mediated Gene Deletion.
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    Chapter 15 The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
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    Chapter 21 Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation
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    Chapter 22 Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo
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    Chapter 46 Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering
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    Chapter 47 Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids
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    Chapter 48 Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells
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    Chapter 57 Gastrointestinal Epithelial Organoid Cultures from Postsurgical Tissues
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    Chapter 58 Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying
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    Chapter 68 Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly
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    Chapter 69 The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism
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    Chapter 70 Efficient Culture of Intestinal Organoids with Blebbistatin
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    Chapter 95 Clinically Amendable, Defined, and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells
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    Chapter 138 Erratum to: Drug Sensitivity Assays of Human Cancer Organoid Cultures
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    Chapter 169 Correction to: The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
Attention for Chapter 22: Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo
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Chapter title
Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo
Chapter number 22
Book title
Organoids
Published in
Methods in molecular biology, April 2017
DOI 10.1007/7651_2017_22
Pubmed ID
Book ISBNs
978-1-4939-7616-4, 978-1-4939-7617-1
Authors

Tong, Kai, Wagle, Mahendra, Guo, Su

Abstract

Stem cells can generate cell fate heterogeneity through asymmetric cell division (ACD). ACD derives from the asymmetric segregation of fate-determining molecules and/or organelles in the dividing cell. Radial glia in the embryonic zebrafish forebrain are an excellent model for studying the molecular mechanisms regulating ACD of stem cells in vertebrates, especially for live imaging concerning in vivo molecular and cellular dynamics. Due to the current difficulty in expressing fluorescent reporter-tagged proteins at physiological levels in zebrafish for live imaging, we have developed an antibody uptake assay to label proteins in live embryonic zebrafish forebrain with high specificity. DeltaD is a transmembrane ligand in Notch signaling pathway in the context of ACD of radial glia in zebrafish. By using this assay, we have successfully observed the in vivo dynamics of DeltaD for studying ACD of radial glia in the embryonic zebrafish forebrain.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 50%
Student > Bachelor 2 17%
Other 1 8%
Student > Ph. D. Student 1 8%
Unknown 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 42%
Agricultural and Biological Sciences 2 17%
Medicine and Dentistry 1 8%
Engineering 1 8%
Unknown 3 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2017.
All research outputs
#20,414,746
of 22,965,074 outputs
Outputs from Methods in molecular biology
#9,918
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Outputs of similar age
#269,452
of 309,738 outputs
Outputs of similar age from Methods in molecular biology
#219
of 273 outputs
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So far Altmetric has tracked 13,137 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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