Chapter title |
Murine Colonic Organoid Culture System and Downstream Assay Applications.
|
---|---|
Chapter number | 8 |
Book title |
Organoids
|
Published in |
Methods in molecular biology, August 2016
|
DOI | 10.1007/7651_2016_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7616-4, 978-1-4939-7617-1
|
Authors |
Yang-Yi Fan, Laurie A. Davidson, Robert S. Chapkin, Fan, Yang-Yi, Davidson, Laurie A., Chapkin, Robert S. |
Abstract |
Colonic organoids, three-dimensional colonic crypts grown in vitro that show realistic microanatomy, have many potential applications for studying physiology, developmental biology, and pathophysiology of intestinal diseases including inflammatory bowel disease and colorectal cancer. Here, we describe detailed protocols for mouse colonic crypt isolation, organoid culture, and downstream applications. Specific culture strategies including growth factor enriched Matrigel and Wnt and R-spondin conditioned media serve as key factors for enhancing the growth and cost efficiency of colonic organoid cultures. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 65 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 18 | 28% |
Researcher | 8 | 12% |
Student > Bachelor | 7 | 11% |
Student > Master | 6 | 9% |
Professor | 3 | 5% |
Other | 7 | 11% |
Unknown | 16 | 25% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 18 | 28% |
Agricultural and Biological Sciences | 8 | 12% |
Medicine and Dentistry | 7 | 11% |
Unspecified | 4 | 6% |
Immunology and Microbiology | 3 | 5% |
Other | 3 | 5% |
Unknown | 22 | 34% |