Chapter title |
Clinically Amendable, Defined, and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells
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---|---|
Chapter number | 95 |
Book title |
Methods in Molecular Biology
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Published in |
Methods in molecular biology, November 2017
|
DOI | 10.1007/7651_2017_95 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7616-4, 978-1-4939-7617-1
|
Authors |
Tomaskovic-Crook, Eva, Crook, Jeremy M. |
Abstract |
Human brain organoids provide opportunities to produce three-dimensional (3D) brain-like tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a protocol for rapid and defined induction of brain organoids from human induced pluripotent stem cells (iPSCs), using commercially available culture and differentiation media and a cheap, easy to handle and clinically approved semisynthetic hydrogel. Importantly, the methodology is uncomplicated, well-defined, and reliable for reproducible and scalable organoid generation, and amendable to principles of current good laboratory practice (cGLP), with the potential for prospective adaptation to current good manufacturing practice (cGMP) toward clinical compliance. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 5 | 14% |
Student > Doctoral Student | 4 | 11% |
Student > Ph. D. Student | 3 | 8% |
Researcher | 3 | 8% |
Student > Postgraduate | 3 | 8% |
Other | 8 | 22% |
Unknown | 10 | 28% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 19% |
Medicine and Dentistry | 6 | 17% |
Agricultural and Biological Sciences | 5 | 14% |
Neuroscience | 2 | 6% |
Social Sciences | 1 | 3% |
Other | 3 | 8% |
Unknown | 12 | 33% |