You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Genotyping
|
---|---|
Chapter number | 14 |
Book title |
Genotyping
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6442-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6440-6, 978-1-4939-6442-0
|
Authors |
Chen, Yuan, Perfect, John R, Perfect, John R., Yuan Chen, John R. Perfect |
Abstract |
Multilocus sequence typing (MLST) has become the preferred method for genotyping many biological species. It can be used to identify major phylogenetic clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. However, conventional MLST is costly and time consuming, which limits its power for genotyping large numbers of samples. Here, we describe a new MLST method that uses next-generation sequencing, a multiplexing protocol, and appropriate analytical software to provide accurate, rapid, and economical MLST genotyping of 96 or more isolates in a single assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 8% |
Unknown | 11 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 33% |
Student > Bachelor | 1 | 8% |
Other | 1 | 8% |
Student > Ph. D. Student | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Other | 0 | 0% |
Unknown | 4 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 17% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Immunology and Microbiology | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Engineering | 1 | 8% |
Other | 0 | 0% |
Unknown | 6 | 50% |