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Mendeley readers
| Chapter title |
Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII
|
|---|---|
| Chapter number | 12 |
| Book title |
Genotyping
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6442-0_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6440-6, 978-1-4939-6442-0
|
| Authors |
Rolf H. A. M. Vossen, Henk P. J. Buermans, Vossen, Rolf H. A. M., Buermans, Henk P. J. |
| Abstract |
Conventional mitochondrial-DNA (MT DNA) sequencing approaches use Sanger sequencing of 20-40 partially overlapping PCR fragments per individual, which is a time- and resource-consuming process. We have developed a high-throughput, accurate, fast, and cost-effective human MT DNA sequencing approach. In this setup we first generate long-range PCR products for two partially overlapping 7.7 and 9.2 kb MT DNA-specific amplicons, add sample-specific barcodes, and sequence these on the PacBio RSII system to obtain full-length MT DNA sequences for genotyping/haplotyping purposes. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 23% |
| Student > Ph. D. Student | 3 | 23% |
| Student > Master | 3 | 23% |
| Researcher | 2 | 15% |
| Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 31% |
| Agricultural and Biological Sciences | 3 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 8% |
| Immunology and Microbiology | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 2 | 15% |