| Chapter title |
Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems
|
|---|---|
| Chapter number | 1 |
| Book title |
Genotyping
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6442-0_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6440-6, 978-1-4939-6442-0
|
| Authors |
Jacqueline Krüger, Dorit Schleinitz, Krüger, Jacqueline, Schleinitz, Dorit |
| Abstract |
Microsatellites are polymorphic DNA loci comprising repeated sequence motifs of two to five base pairs which are dispersed throughout the genome. Genotyping of microsatellites is a widely accepted tool for diagnostic and research purposes such as forensic investigations and parentage testing, but also in clinics (e.g. monitoring of bone marrow transplantation), as well as for the agriculture and food industries. The co-amplification of several short tandem repeat (STR) systems in a multiplex reaction with simultaneous detection helps to obtain more information from a DNA sample where its availability may be limited. Here, we introduce and describe this commonly used genotyping technique, providing an overview on available resources on STRs, multiplex design, and analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 28% |
| Student > Master | 3 | 10% |
| Researcher | 3 | 10% |
| Student > Ph. D. Student | 3 | 10% |
| Other | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 8 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 34% |
| Agricultural and Biological Sciences | 3 | 10% |
| Engineering | 2 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 4 | 14% |
| Unknown | 8 | 28% |