Chapter title |
Oncogene-Induced Senescence
|
---|---|
Chapter number | 8 |
Book title |
Oncogene-Induced Senescence
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6670-7_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6668-4, 978-1-4939-6670-7
|
Authors |
Narita, Masako, Narita, Masashi, Masako Narita, Masashi Narita |
Abstract |
Oncogene-induced senescence (OIS) is a highly dynamic process, involving several different effector mechanisms, the multitude and combination of which likely determines the quality of the phenotype (Pérez-Mancera et al., Nat Rev Cancer 14:547-558, 2014). Autophagy, a cellular degradation process, has been proposed to be one of these senescence effectors, although its functional relevance seems highly context dependent (Hoare et al., Semin Cancer Biol 21:397-404, 2011). A number of methods for monitoring autophagy are available, and several excellent protocols have been published in this journal (Klionsky et al., Autophagy 8:445-544, 2012; Tooze et al., Methods Mol Biol 1270:155-165, 2015; Tabata et al., Methods Mol Biol 931:449-466, 2013; Young and Tooze, Methods Mol Biol 445:147-157, 2008). The same principles apply to models of OIS in culture. Thus, in this chapter, we describe how to generate OIS cells using human diploid fibroblasts (HDFs), the best-characterized cell model of OIS, and how to detect autophagy, particularly focusing on immunofluorescence methods. |
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