| Chapter title |
Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells
|
|---|---|
| Chapter number | 10 |
| Book title |
Oncogene-Induced Senescence
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6670-7_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6668-4, 978-1-4939-6670-7
|
| Authors |
Konstantinos Evangelou, Vassilis G. Gorgoulis, Evangelou, Konstantinos, Gorgoulis, Vassilis G. |
| Abstract |
The Sudan-Black-B (SBB) histochemical stain is well known to specifically react against lipofuscin, an aggregate of oxidized proteins, lipids, and metals. Lipofuscin is related to many ageing processes. It is also known to accumulate in senescent cells. We recently proved that lipofuscin detection, when applying the SBB staining, is highly specific for the visualization of senescent cells. Here, we present in detail this SBB method that can detect senescent cells in any material, irrespective of its preparation. This provides unique advantages not only in understanding physiological processes and the pathophysiology of various diseases but also in estimating the response to therapeutic interventions. |
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|---|---|---|
| Unknown | 94 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Bachelor | 18 | 19% |
| Researcher | 7 | 7% |
| Student > Master | 7 | 7% |
| Student > Doctoral Student | 4 | 4% |
| Other | 9 | 10% |
| Unknown | 28 | 30% |
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|---|---|---|
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| Environmental Science | 3 | 3% |
| Other | 13 | 14% |
| Unknown | 29 | 31% |