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In Vitro Mutagenesis Protocols

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Cover of 'In Vitro Mutagenesis Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension
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    Chapter 2 In Vitro Mutagenesis of Brucella Species
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    Chapter 3 Random Mutagenesis Strategies for Campylobacter and Helicobacter Species
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    Chapter 4 Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artificial Chromosomes
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    Chapter 5 An Efficient Protocol for VZV BAC-Based Mutagenesis
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    Chapter 6 A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxb1 Integrase
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    Chapter 7 In Vitro Mutagenesis Protocols
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    Chapter 8 A rapid and versatile PCR-based site-directed mutagenesis protocol for generation of mutations along the entire length of a cloned cDNA.
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    Chapter 9 Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP)
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    Chapter 10 Insertion and Deletion Mutagenesis by Overlap Extension PCR
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    Chapter 11 In Vitro Mutagenesis Protocols
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    Chapter 12 A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins
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    Chapter 13 Amplification of Orthologous Genes Using Degenerate Primers
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    Chapter 14 Computational Evaluation of Protein Stability Change upon Mutations
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    Chapter 15 Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations
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    Chapter 16 Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins
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    Chapter 17 Massive Mutagenesis ® : High-Throughput Combinatorial Site-Directed Mutagenesis
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    Chapter 18 Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High-Throughput Screening
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    Chapter 19 Ribosome Display for Rapid Protein Evolution by Consecutive Rounds of Mutation and Selection
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    Chapter 20 Fine-Tuning Enzyme Activity Through Saturation Mutagenesis
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    Chapter 21 Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties
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    Chapter 22 Site-Directed Mutagenesis for Improving Biophysical Properties of V H Domains
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    Chapter 23 Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells
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    Chapter 24 Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example
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    Chapter 25 Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems
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    Chapter 26 Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases
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    Chapter 27 Random-Scanning Mutagenesis
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    Chapter 28 Easy Two-Step Method for Randomizing and Cloning Gene Fragments
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    Chapter 29 Random Mutagenesis Using a Mutator Strain
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    Chapter 30 En Passant Mutagenesis: A Two Step Markerless Red Recombination System
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In Vitro Mutagenesis Protocols
Published by
Humana Press, August 2010
DOI 10.1007/978-1-60761-652-8
978-1-60761-651-1, 978-1-60761-652-8

Braman, Jeff

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Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 58 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 15 26%
Researcher 9 16%
Student > Ph. D. Student 9 16%
Student > Master 6 10%
Professor > Associate Professor 3 5%
Other 4 7%
Unknown 12 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 36%
Agricultural and Biological Sciences 8 14%
Immunology and Microbiology 3 5%
Chemical Engineering 2 3%
Environmental Science 2 3%
Other 9 16%
Unknown 13 22%