Chapter title |
N -Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF
|
---|---|
Chapter number | 8 |
Book title |
Carbohydrate-Based Vaccines
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2874-3_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2873-6, 978-1-4939-2874-3
|
Authors |
René Hennig, Erdmann Rapp, Robert Kottler, Samanta Cajic, Matthias Borowiak, Udo Reichl, Hennig, René, Rapp, Erdmann, Kottler, Robert, Cajic, Samanta, Borowiak, Matthias, Reichl, Udo |
Abstract |
The ongoing threat of pathogens, increasing resistance against antibiotics, and the risk of fast spreading of infectious diseases in a global community resulted in an intensified development of vaccines. Antigens used for vaccination comprise a wide variety of macromolecules including glycoproteins, lipopolysaccharides, and complex carbohydrates. For all of these antigens the sugar composition plays a crucial role for immunogenicity and protective efficacy of the vaccine. Here, we provide a protocol for N-glycosylation fingerprinting utilizing high performance multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF) technology. The method described, enables to analyze the N-glycosylation of specific proteins out of a complex sample or even the total of all N-glycans contained in such a sample. The protocol is exemplarily demonstrated for N-glycosylation fingerprinting of cell culture-derived influenza A and B viruses and their major antigens, the membrane glycoproteins hemagglutinin and neuraminidase. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 26% |
Student > Master | 4 | 17% |
Student > Bachelor | 2 | 9% |
Researcher | 2 | 9% |
Professor > Associate Professor | 2 | 9% |
Other | 3 | 13% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 39% |
Chemistry | 2 | 9% |
Agricultural and Biological Sciences | 2 | 9% |
Medicine and Dentistry | 2 | 9% |
Unspecified | 1 | 4% |
Other | 1 | 4% |
Unknown | 6 | 26% |