| Chapter title |
Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods
|
|---|---|
| Chapter number | 13 |
| Book title |
Carbohydrate-Based Vaccines
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2874-3_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2873-6, 978-1-4939-2874-3
|
| Authors |
Stephen E. Harding, Ali Saber Abdelhameed, Richard B. Gillis, Gordon A. Morris, Gary G. Adams, Harding, Stephen E., Abdelhameed, Ali Saber, Gillis, Richard B., Morris, Gordon A., Adams, Gary G. |
| Abstract |
Hydrodynamic methods are relevant for the characterization of carbohydrates such as capsular bacterial polysaccharides or glycoconjugates in solution. This chapter focuses on the following hydrodynamic methods: sedimentation velocity analytical ultracentrifugation (SV AUC), dynamic light scattering (DLS), sedimentation equilibrium analytical ultracentrifugation (SE AUC), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS), and capillary viscometry-intrinsic viscosity measurement. The chapter highlights the general principle of these five methods, describes experimental details, and specifies advances in the last years. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 27% |
| Unspecified | 1 | 9% |
| Lecturer > Senior Lecturer | 1 | 9% |
| Other | 1 | 9% |
| Student > Master | 1 | 9% |
| Other | 2 | 18% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 36% |
| Chemistry | 2 | 18% |
| Unspecified | 1 | 9% |
| Sports and Recreations | 1 | 9% |
| Computer Science | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |