Chapter title |
Isolation and Analysis of Exosomal MicroRNAs from Ovarian Follicular Fluid
|
---|---|
Chapter number | 4 |
Book title |
MicroRNA Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7601-0_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7600-3, 978-1-4939-7601-0
|
Authors |
Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, Gerrit J. Bouma, Silveira, Juliano Da, Andrade, Gabriella M., Perecin, Felipe, Meireles, Flávio Vieira, Winger, Quinton A., Bouma, Gerrit J. |
Abstract |
Mammalian ovarian follicular growth is characterized by development of a large fluid filled antrum that separates mural granulosa cells and cumulus cells. Extensive communication between the different cell types is necessary for maturation of a developmentally competent oocyte. Here, we describe an approach for the isolation of cell-secreted exosomes from ovarian follicular fluid, identification of small RNAs (i.e., microRNAs) in exosomes, labeling of exosomes, and examining cell uptake of exosomes by follicular cells. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 37 | 100% |
Demographic breakdown
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Student > Bachelor | 6 | 16% |
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Researcher | 2 | 5% |
Other | 3 | 8% |
Unknown | 11 | 30% |
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Other | 1 | 3% |
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