Chapter title |
Surface Plasmon Resonance Method to Evaluate Anti-citrullinated Protein/Peptide Antibody Affinity to Citrullinated Peptides
|
---|---|
Chapter number | 23 |
Book title |
Peptide Antibodies
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2999-3_23 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2998-6, 978-1-4939-2999-3
|
Authors |
Feliciana Real-Fernández, Giada Rossi, Filomena Panza, Federico Pratesi, Paola Migliorini, Paolo Rovero, Real-Fernández, Feliciana, Rossi, Giada, Panza, Filomena, Pratesi, Federico, Migliorini, Paola, Rovero, Paolo |
Abstract |
Surface plasmon resonance (SPR) technique is extremely interesting in immunology because it has the potential to directly visualize biomolecular interactions in real-time monitoring antibody affinity, one of the parameters affecting pathogenicity in autoimmune diseases. Herein we describe the affinity evaluation of anti-citrullinated peptide antibodies (ACPA) to a peptide-based biosensor by SPR. The method describes the purification of ACPA isolated from rheumatoid arthritis (RA) patients using affinity columns, the strategy employed for the immobilization of citrullinated peptides onto a sensor chip, and the evaluation of the specific binding of purified ACPA to immobilized peptides. |
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