You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies
|
|---|---|
| Chapter number | 15 |
| Book title |
Peptide Antibodies
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2999-3_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2998-6, 978-1-4939-2999-3
|
| Authors |
Hui Ma, Richard O’Kennedy, Ma, Hui, O’Kennedy, Richard |
| Abstract |
The purification of peptide antibodies (e.g., IgG, IgY, scFv, and Fab) are described in this chapter. Affinity chromatographic purification, a very convenient and effective antibody purification strategy, is used to isolate peptide antibodies based on specific binding, i.e., binding of the antibody to a column on which its specific ligand is immobilized with subsequent elution of the purified antibody. In addition, the application of purification methods based on the use of proteins A, G, and L, each of which bind to specific domains on an antibody/fragment, or the use of specific tags (e.g., histidine and biotin) attached to antibodies or antigens are also described. |
Mendeley readers
The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 14% |
| Student > Bachelor | 2 | 14% |
| Researcher | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Professor | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 3 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Other | 3 | 21% |
| Unknown | 5 | 36% |