Chapter title |
Generation of TCR-Like Antibodies Using Phage Display
|
---|---|
Chapter number | 17 |
Book title |
Peptide Antibodies
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2999-3_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2998-6, 978-1-4939-2999-3
|
Authors |
Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung, Santich, Brian H., Liu, Hong, Liu, Cheng, Cheung, Nai-Kong V. |
Abstract |
The adaptive immune response against cancer consists of two arms: the humoral response from B cells, and the cell-mediated response from T cells. The humoral response has the advantage of diversity, theoretically recognizing antigens of any type (sugar, protein, lipid, etc.), but is generally limited to surface-expressed targets. T cells on the other hand, can recognize intracellular targets, but only if they are proteins, and presented as small peptide fragments on major histocompatibility complex (MHC) cell surface antigens. However, with advances in protein engineering and phage display, it has become feasible to quickly identify and generate antibodies or single-chain variable fragments against peptide-MHC, thus bridging the two arms, and allowing for recognition, identification, and effector responses against cells expressing intracellular targets. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 4 | 21% |
Student > Master | 3 | 16% |
Student > Doctoral Student | 2 | 11% |
Student > Bachelor | 2 | 11% |
Student > Ph. D. Student | 1 | 5% |
Other | 3 | 16% |
Unknown | 4 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 21% |
Agricultural and Biological Sciences | 4 | 21% |
Immunology and Microbiology | 2 | 11% |
Unspecified | 1 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Other | 3 | 16% |
Unknown | 4 | 21% |