You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Identification of S-Nitrosothiols by the Sequential Cysteine Blocking Technique
|
|---|---|
| Chapter number | 14 |
| Book title |
Plant Nitric Oxide
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3600-7_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3598-7, 978-1-4939-3600-7
|
| Authors |
Homem, Rafael A., Le Bihan, Thierry, Yu, Manda, Loake, Gary J., Rafael A. Homem, Thierry Le Bihan, Manda Yu, Gary J. Loake, Bihan, Thierry Le |
| Abstract |
Here, we describe a procedure for the identification of S-nitrosothiols that has been used in our laboratory to study the roles of protein S-nitrosylation in the immune responses of Arabidopsis thaliana and other organisms. It employs a modified version of the biotin-switch technique, which we termed the sequential cysteine blocking technique, encompassing the sequential redox-blocking of recombinant proteins followed by LC-MS/MS analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer > Senior Lecturer | 2 | 40% |
| Researcher | 2 | 40% |
| Student > Ph. D. Student | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 60% |
| Agricultural and Biological Sciences | 1 | 20% |
| Engineering | 1 | 20% |