Chapter title |
Bovine Granulosa Cell Culture
|
---|---|
Chapter number | 8 |
Book title |
Epithelial Cell Culture
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8600-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8599-9, 978-1-4939-8600-2
|
Authors |
Bushra T. Mohammed, F. Xavier Donadeu, Mohammed, Bushra T., Donadeu, F. Xavier |
Abstract |
Culture of granulosa cells has for long provided a useful tool to understand the molecular processes underlying ovarian follicle development. Among all species investigated, cattle have become an excellent model for in vitro studies on follicular biology, both because of their resemblance with humans in terms of follicular biology and the importance of reproductive failure as a cause of lost productivity in the dairy industry. In this chapter, we describe up-to-date methods for the harvesting of granulosa cells from bovine ovaries collected post-mortem, as well as procedures for both culturing granulosa cells in an undifferentiated state and inducing their luteinization in vitro, and for the efficient transfection of granulosa cells with oligonucleotide sequences for the purpose of investigating the function of specific genes in vitro. |
X Demographics
Geographical breakdown
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 4 | 17% |
Student > Master | 4 | 17% |
Researcher | 2 | 8% |
Student > Bachelor | 2 | 8% |
Student > Doctoral Student | 2 | 8% |
Other | 2 | 8% |
Unknown | 8 | 33% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 6 | 25% |
Biochemistry, Genetics and Molecular Biology | 6 | 25% |
Veterinary Science and Veterinary Medicine | 2 | 8% |
Unspecified | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 0 | 0% |
Unknown | 8 | 33% |