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Mendeley readers
| Chapter title |
Ovine Granulosa Cells Isolation and Culture to Improve Oocyte Quality
|
|---|---|
| Chapter number | 10 |
| Book title |
Epithelial Cell Culture
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8600-2_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8599-9, 978-1-4939-8600-2
|
| Authors |
Giovanni Giuseppe Leoni, Salvatore Naitana, Leoni, Giovanni Giuseppe, Naitana, Salvatore |
| Abstract |
The functional cooperation between the oocyte and its surrounding granulosa cells is mandatory to oocyte growth and acquisition of developmental competence in mammalian species. Availability of in vitro methods for culture granulosa cells is important in understanding the biology of these cells and the response of maturing oocyte to in vitro culture and co-culture. Using the ovine as a model the ensuing chapter describes methods for primary culture of ovine granulosa cells and their co-culture with theca cells or oocytes that allow to mimic the molecular interactions between somatic cells and maturing oocyte and improve oocyte quality. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 29% |
| Researcher | 2 | 29% |
| Student > Ph. D. Student | 1 | 14% |
| Other | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 57% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unknown | 1 | 14% |