Chapter title |
Murine and Human Mammary Cancer Cell Lines: Functional Tests
|
---|---|
Chapter number | 17 |
Book title |
Epithelial Cell Culture
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8600-2_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8599-9, 978-1-4939-8600-2
|
Authors |
Paolo Accornero, Eugenio Martignani, Silvia Miretti, Mario Baratta, Accornero, Paolo, Martignani, Eugenio, Miretti, Silvia, Baratta, Mario |
Abstract |
The biological characterization of mammary cancer cells is a prerequisite that helps the scientist understand some aspect of tumor biology. Once isolated from the tumor, cells are subjected to multiple tests that dissect their ability to growth, migrate, degrade the surrounding stroma, produce 3-dimensional structures and differentiate. Targeted inhibitors, when added to these tests, are used to unravel how specific growth factors, receptors, and intracellular translational pathways promote the ability of mammary tumor cells to achieve their biological behavior. Herein we describe a set of techniques used to put in focus the biological capacities in mammary cancer cells. When the characterization of a biological trait (e.g., proliferation) is assessable by multiple assays, we will limit the description to only one technique, possibly the easier to manage and that requires minimal laboratory equipment. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 33% |
Professor | 1 | 17% |
Lecturer | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 33% |
Unspecified | 1 | 17% |
Veterinary Science and Veterinary Medicine | 1 | 17% |
Unknown | 2 | 33% |