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Mendeley readers
Chapter title |
Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids
|
---|---|
Chapter number | 15 |
Book title |
Epithelial Cell Culture
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8600-2_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8599-9, 978-1-4939-8600-2
|
Authors |
Emma Teal, Nina Bertaux-Skeirik, Jayati Chakrabarti, Loryn Holokai, Yana Zavros, Teal, Emma, Bertaux-Skeirik, Nina, Chakrabarti, Jayati, Holokai, Loryn, Zavros, Yana |
Abstract |
Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 25% |
Other | 2 | 25% |
Student > Ph. D. Student | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 25% |
Nursing and Health Professions | 1 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Engineering | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |