| Chapter title |
Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro
|
|---|---|
| Chapter number | 7 |
| Book title |
Experimental Models of Cardiovascular Diseases
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-8597-5_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-8596-8, 978-1-4939-8597-5
|
| Authors |
Przemek A. Gorski, Changwon Kho, Jae Gyun Oh, Gorski, Przemek A., Kho, Changwon, Oh, Jae Gyun |
| Abstract |
In vitro measurements of cardiomyocyte contractility and Ca2+ handling have been used as a platform for determining physiological consequence of various genetic manipulations and identifying potential therapeutic targets for the treatment of heart failure. The Myocyte Calcium and Contractility System (IonOptix) offers a simultaneous trace of sarcomere movements and changes of intracellular Ca2+ levels in a single cardiomyocyte. Herein, we describe a modified protocol for the isolation of adult cardiomyocytes from murine hearts and provide a step-by-step description on how to analyze cardiomyocyte Ca2+ transient and contractility data collected using the IonOptix system. In our modified protocol, we recommend a novel cannulation technique which simplifies this difficult method and leads to improved viability of isolated cardiomyocytes. In addition, a comprehensive analysis of intracellular Ca2+ handling, SR Ca2+ load, myofilament Ca2+ sensitivity, and cardiomyocyte contractility is described in order to provide important insights into myocardial mechanics. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 13% |
| Student > Bachelor | 4 | 10% |
| Researcher | 4 | 10% |
| Student > Master | 4 | 10% |
| Student > Doctoral Student | 3 | 8% |
| Other | 4 | 10% |
| Unknown | 16 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 30% |
| Medicine and Dentistry | 5 | 13% |
| Engineering | 4 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 5% |
| Immunology and Microbiology | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 15 | 38% |