Chapter title |
Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes Using Embryoid Bodies
|
---|---|
Chapter number | 6 |
Book title |
Experimental Models of Cardiovascular Diseases
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8597-5_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8596-8, 978-1-4939-8597-5
|
Authors |
Takeshi Hatani, Kenji Miki, Yoshinori Yoshida, Hatani, Takeshi, Miki, Kenji, Yoshida, Yoshinori |
Abstract |
Specific cell lineages differentiated from human induced pluripotent stem cells (iPSCs) are promising sources for cell replacement therapy and are useful biomedical research tools for research on disease mechanisms and drug discovery. Among the different lineages, cardiac lineage has been one of the most efficiently differentiated through several established protocols. In this chapter, we describe our reproducible and highly efficient methodology for differentiating iPSCs into cardiomyocytes using embryoid bodies. We also describe methods to dissociate iPSC-derived cardiomyocytes and to evaluate iPSC-derived cardiomyocytes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 33% |
Student > Bachelor | 1 | 7% |
Researcher | 1 | 7% |
Professor > Associate Professor | 1 | 7% |
Student > Postgraduate | 1 | 7% |
Other | 0 | 0% |
Unknown | 6 | 40% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 27% |
Biochemistry, Genetics and Molecular Biology | 2 | 13% |
Medicine and Dentistry | 2 | 13% |
Business, Management and Accounting | 1 | 7% |
Unknown | 6 | 40% |